The invention relates to a non-covalent conjugate of a biological medicine, a preparation method and use of a pharmaceutical composition formed from the non-covalent conjugate. The biological medicine is conjugated to a high-molecular polymer at high concentration so as to form the non-covalent conjugate; the biological medicine is dissociated and released from the high-molecular polymer at low concentration, so that the pharmacological activity of the biological medicine takes effect. The method disclosed by the invention is characterized by being not only capable of stably reserving medicines and reducing the immunogenicity of medicines but also capable of relieving diffusion velocity and clearing velocity from blood, prolonging the time of keeping the effective treatment concentration of medicinal plasma and the half-life period, and basically eliminating medicine burst release. The invention further relates to use of the pharmaceutical composition containing the non-covalent conjugate of the biological medicine.