The invention provides a cleaning device of a sampling needle. The cleaning device comprises a cleaning pool, the cleaning pool is provided with a main runner allowing the sampling needle to be inserted and a spraying hole with a liquid outlet, and the liquid outlet is communicated to the main runner and used for cleaning the outer wall of a tip of the sampling needle passing the liquid outlet of the spraying hole and the outer wall, needing to be cleaned, of a sampling needle body. By arranging the main runner and the spraying hole, spraying cleaning is performed twice on the outer wall of the sampling needle; during spraying of the first time, water ejected by the spraying hole directly flushes the outer wall of the tip of the sampling needle in a short-distance manner, and most samples or reagents left on the outer wall of the tip can be flushed away; during spraying of the second time, the outer wall, needing to be cleaned, of the sampling needle body and the outer wall of the sampling needle are cleaned through a cleaning liquid output by the spraying hole, so that when cleaning height range of the outer wall of the sampling needle is large, the outer wall of the tip of the sampling needle can be cleaned effectively, and pollutant carrying probability of the tip of the sampling needle is lowered. The invention further provides a cleaning method of the sampling needle.