基本信息:
- 专利标题： 一种细菌漆酶突变体蛋白、其重组表达质粒、转化的工程菌株及其发酵制备方法
- 专利标题（英）：Bacterial laccase mutant protein, recombinant expression plasmid, transformed engineered strain and fermentation preparation method thereof
- 申请号：CN201310655726.4 申请日：2013-12-05
- 公开(公告)号：CN104087560A 公开(公告)日：2014-10-08
- 发明人： 方泽民 , 肖亚中 , 常飞 , 周鹏 , 张学成 , 房伟 , 袁璟
- 申请人： 安徽大学
- 申请人地址： 安徽省合肥市经济技术开发区九龙路111号
- 专利权人： 安徽大学
- 当前专利权人： 安徽大学
- 当前专利权人地址： 安徽省合肥市经济技术开发区九龙路111号
- 代理机构： 安徽省合肥新安专利代理有限责任公司
- 代理人： 何梅生; 王伟
- 生物保藏信息： CCTCC NO：M2013598 2013.11.24
- 主分类号： C12N9/02
- IPC分类号： C12N9/02 ; C12N15/53 ; C12N15/70 ; C12N1/21 ; C12R1/19
The invention discloses a bacterial laccase mutant protein, which is characterized in that the mutant protein amino acid sequence is obtained by deletion mutation of the 323rd glycine residue to the 332rd glycine residue in the bacterial laccase amino acid sequence shown as SEQ ID No.1. Through a genetic engineering reconstruction method, a stability improved bacterial laccase protein coding gene, its expression plasmid and engineered bacteria can be obtained, and after large-scale fermentation and induced expression of the engineered bacteria, the stability improved bacterial laccase protein can be obtained. According to the invention, the marine uncultured microorganism source bacterial laccase Lac15 is taken as the foundation, and by means of genetic engineering reconstruction, mutant gene can be obtained. At the same time, a recombinant escherichia coli is employed to conduct high-density culture for high-efficiency expression of the bacterial laccase mutant protein. According to the invention, the stability and yield of the bacterial laccase are greatly improved.
公开/授权文献:
- CN104087560B 一种细菌漆酶突变体蛋白、其重组表达质粒、转化的工程菌株及其发酵制备方法 公开/授权日：2016-10-19