The invention belongs to the technical field of rat fixing devices, and particularly relates to a rat fixing device for acupuncture. The rat fixing device mainly solves the problems that an existing rat fixing device is small in acupuncture range, poor in regulation degree and difficult to wash. According to the rat fixing device for acupuncture, a head fixing device body and a body fixing device body are arranged on a base, the body fixing device body is fixed onto the base through four supporting legs, the rear end of the head fixing device body is connected with the front end of the body fixing device body, and an ear cover is arranged on the head fixing device body. The body fixing device body is composed of a bottom plate, a left side plate, a right side plate, an air bag, an excretion tank and a buttock fixing plate, the left side plate and the right side plate are arranged on the left side and the right side of the bottom plate respectively, a tooth-shaped buckle is arranged on the left side plate, a buckle base is arranged on the inner surface of the right side plate, an excretion hole is formed in the rear portion of the bottom plate, the excretion tank is arranged below the excretion hole in the rear portion of the bottom plate, an inserting groove is formed in the rear end of the bottom plate, the buttock fixing plate is arranged in the inserting groove, and a tail hole is formed in the buttock fixing plate.