The invention discloses orientation groove micro/nano-fibres and a preparation method for the same. The orientation groove micro/nano-fibres comprise common groove micro/nano-fibres or beaded groove micro/nano-fibres, and the diameters of the fibres are 50 nanometres to 10 microns. The preparation method for the same comprises the following steps of: preparing polymer solution containing a high-volatility solvent and a low-volatility solvent, and preparing micro/nano-fibres by virtue of an electrospinning technique; preparing micro/nano-fibres with different groove structures, for example, different groove quantities, groove depths, groove distances and the like by adjusting process parameters of a solvent ratio, a solution concentration, a relative humidity and the like. The orientation groove micro/nano-fibres disclosed by the invention have the advantages of large specific surface area, high porosity and the like, and have a wide application prospect in the fields of superhydrophobicity, filter, tissue engineering and the like; the preparation method disclosed by the invention is capable of directly preparing the orientation groove micro/nano-fibres without the need of any aftertreatment process, simple to operate, stable in process, low in cost, and capable of realizing batch production.