基本信息:
- 专利标题： 包含聚合物单元的聚合物的分析
- 专利标题（英）：Analysis of a polymer comprising polymer units
- 申请号：CN201280057564.8 申请日：2012-09-21
- 公开(公告)号：CN104066850A 公开(公告)日：2014-09-24
- 发明人： 斯图尔特·威廉·里德 , 加文·哈珀 , 克莱夫·加文·布朗 , 詹姆斯·安东尼·克拉克 , 安德鲁·约翰·赫伦
- 申请人： 牛津楠路珀尔科技有限公司
- 申请人地址： 英国牛津
- 专利权人： 牛津楠路珀尔科技有限公司
- 当前专利权人： 牛津楠路珀尔科技有限公司
- 当前专利权人地址： 英国牛津
- 代理机构： 北京康信知识产权代理有限责任公司
- 代理人： 余刚; 张英
- 优先权： 61/538,721 2011.09.23 US; 61/617,880 2012.03.30 US
- 国际申请： PCT/GB2012/052343 2012.09.21
- 国际公布： WO2013/041878 EN 2013.03.28
- 进入国家日期： 2014-05-22
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/487 ; G06F19/22 ; B82Y15/00
A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.
公开/授权文献:
- CN104066850B 包含聚合物单元的聚合物的分析 公开/授权日：2017-11-10