The invention provides an electron microscope biologic sample batch tissue treatment device which consists of a sample basket, handles, a solution tank, a tank cover, cell filtering nets and pressing rings, wherein the sample basket is provided with numbered grids which are arranged in a matrix; the bottom of the sample basket is of a sieve structure; the sample basket is put into the solution tank; the cell filtering nets are arranged inside the grids; the sizes of the filtering nets are accordant with those of the numbered grids; the pressing rings are arranged at the bottoms of the filtering nets. The device is reasonable in design, convenient to manufacture and low in manufacturing cost, when the device is used, the experiment operation time is greatly shortened, various electron microscope biologic samples, including blocky tissue samples and dispersed cell samples, can be prepared in batches, the use cost is lowered, parallel experiments are achieved, very high feasibility is achieved, and the batch treatment of biologic samples is implemented substantially.