The invention provides an experimental device for shale movable oil ration determination, the experimental device comprises a formation water container, a high-pressure constant-pressure pump, a heating furnace, a high-pressure kettle and an oil collecting bottle, wherein the high-pressure constant-pressure pump is connected between the formation water container and the high-pressure kettle to inject fluid in the formation water container into a shale sample in the high-pressure kettle and exert and control pressure equivalent to the fluid pressure under formation condition, the high-pressure kettle is arranged in the heating furnace, the heating surface is used for heating the high-pressure kettle and controlling the temperature equivalent to that under the formation condition; the oil collecting bottle is connected with the high-pressure kettle and used for collecting the fluid produced by compressing and decompressing the shale sample in the high-pressure kettle. The experimental device for shale movable oil ration determination is capable of stimulating the shale oil production feature at the high-temperature high-pressure condition underground, the property and the quantity of the experimentally produced shale oil are closer to those of the underground time condition, the determined shale movable oil ratio and proportion can be directly applied to the resource evaluation of the shale oil and the guidance of exploration of the shale oil.