基本信息:
- 专利标题： 微阵列芯片检测中核苷酸序列的颗粒标记方法
- 专利标题（英）：Particle labeling method of nucleotide sequence in micro-array chip detection
- 申请号：CN201310651906.5 申请日：2013-12-05
- 公开(公告)号：CN103760355A 公开(公告)日：2014-04-30
- 发明人： 李阳 , 张冠斌 , 蒋迪 , 项光新 , 邢婉丽 , 程京
- 申请人： 博奥生物有限公司 , 清华大学
- 申请人地址： 北京市昌平区生命科学园路18号
- 专利权人： 博奥生物有限公司,清华大学
- 当前专利权人： 北京博奥晶典生物技术有限公司,清华大学
- 当前专利权人地址： 北京市昌平区生命科学园路18号
- 代理机构： 北京纪凯知识产权代理有限公司
- 代理人： 关畅; 陈晓庆
- 主分类号： G01N33/58
- IPC分类号： G01N33/58 ; C12Q1/68
The invention discloses a particle labeling method of a nucleotide sequence in micro-array chip detection. The method for detecting target molecules in a micro-array chip comprises the following steps: coating the surface of a particle with a plurality of function groups, and marking the obtained particle as a functionalized particle; and labeling all the target molecules with modification groups, and marking the obtained target molecules as modified target molecules, wherein the function groups can be combined with the modification groups, and the functionalized particle and a luminescence group enable all the modified target molecules to shine in order to realize the labeling of the target molecules. The fluorescent labeling of the target molecules can be directly or indirectly carried out through the disclosed particle labeling method, and the labeling method can greatly reduce the fluorescence detection cost and also avoids the inhibition effect of the PCR enriched target nucleic acid molecule labeling in traditional fluorescent labeling methods.
公开/授权文献:
- CN103760355B 微阵列芯片检测中核苷酸序列的颗粒标记方法 公开/授权日：2015-09-16