The invention relates to a display screen detection device, in particular to a method and device for OLED screen pixel defect detection. The method includes the steps of placing an OLED screen on a detection platform, initializing a detection system, obtaining information, including an original image, of the detected OLED screen, conducting preprocessing on the original image when the original image is received, conducting rotating correction on a preprocessed original image when the preprocessed original image is received, conducting iteration processing on an original image obtained through rotating correction when the original image obtained through rotating corrosion is received, starting to conduct image segmentation on an original image obtained through iteration processing when the original image obtained through iteration processing is received, starting to compare images obtained through segmentation with the complete image to find out whether defects exist or not according to the received images obtained through segmentation, if not, continuing to detect the next OLED screen, and if yes, giving an alarm and positioning the defects.