基本信息:
- 专利标题： 一种可并行检测多种弧菌的基因芯片及其检测方法
- 专利标题（英）：Gene chip capable of simultaneously detecting various vibrios and method for detecting vibrios
- 申请号：CN201310437410.8 申请日：2013-09-23
- 公开(公告)号：CN103451310A 公开(公告)日：2013-12-18
- 发明人： 曲凌云 , 朱鹏飞 , 李壹 , 田欣欣 , 王琛
- 申请人： 国家海洋局第一海洋研究所
- 申请人地址： 山东省青岛市崂山区仙霞岭路6号
- 专利权人： 国家海洋局第一海洋研究所
- 当前专利权人： 国家海洋局第一海洋研究所
- 当前专利权人地址： 山东省青岛市崂山区仙霞岭路6号
- 代理机构： 青岛海昊知识产权事务所有限公司
- 代理人： 曾庆国
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12Q1/04 ; C40B40/06 ; C40B50/18 ; C12R1/63
The invention relates to a gene chip capable of simultaneously detecting various vibrios and a method for detecting the vibrios, and provides species-specific genes of four common pathogenic vibrios in the aquatic environment to encode an oligonucleotide probe sequence of an hsp60 gene and an oligonucleotide probe sequence of a toxR gene, wherein the four common pathogenic vibrios include vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus and vibrio haeveyi. On the basis of the base complementary and pairing principle, by combining the method with an optimized ordinary PCR and fluorescence labeled PCR reaction system, the aim of detection is achieved through oligonucleotide probes fixed to a solid-phase aldehyde chip. The invention provides the detecting method which is high in flux, specificity and sensitivity, rapid and efficient for the bottleneck of a current method for detecting pathogenic microorganisms.
公开/授权文献:
- CN103451310B 一种可并行检测多种弧菌的基因芯片及其检测方法 公开/授权日：2015-02-18