The invention discloses a microwave extraction method of canned fish seasoning liquid, which comprises the following steps of mixing the following components in parts by weight: 1-5 parts of anise, 1-5 parts of pepper, 1-2 parts of fennel, 1-2 parts of clove, 1-2 parts of liquorice, 0.5-1.5 parts of radix angelicae, 0.5-1.5 parts of bay leaves, 0.5-1.5 parts of Bibo, 0.5-1.5 parts of cortex cinnamomi, 0.25-0.75 part of rhizoma kaempferiae, 0.25-0.75 part of radix aucklandiae, 0.25-0.75 part of kaempferia galangal, 0-3 parts of hawthorn and 0-3 parts of black pepper; grinding; and performing microwave extraction to obtain the canned fish seasoning liquid. According to the method disclosed by the invention, by adopting a microwave dynamic circulating reverse flow method to extract active ingredients in a Chinese herbal medicine, the prepared canned fish seasoning liquid integrates the essences of multiple traditional Chinese medicines, and has good nourishing and seasoning functions; and moreover, the canned fish seasoning liquid has a strong, pure and lasting aroma, and has the functions of strengthening the spleen and promoting appetite, removing the mutton smell and improving freshness and eliminating fishy smell and peculiar smell.