The invention discloses heat transfer printing glue for glass, and a preparation method of the heat transfer printing glue. The heat transfer printing glue for glass comprises the following ingredients in parts by weight: 30-35 parts of E-44 epoxy resin, 12-15 parts of E-12 epoxy resin, 5-8 parts of divinylbenzene-compounded phenol polyoxyethylene ether, 8-10 parts of aluminic acid ester coupling agent DL-411, 1-2 parts of scorch retarder, 8-12 parts of polytetramethylene-oxide glycol, 10-12 parts of ethyl acetate, 2-3 parts of cumene hydroperoxide, 5-8 parts of 4,4'-diphenylmethane diisocyanate, 1-2 parts of antioxidant 1010, 1-2 parts of hydroxyethyl heptadecenyl glyoxalidine, 1-2 parts of smoke sheet rubber, 1-2 parts of ethidene diamine, 40-50 parts of acetone, and 70-80 parts of xylene. Compared with the prior art, the heat transfer printing glue is used for transfer printing for patterns on glass products, and has the advantages of being firm to bind, not easy to damage, fall and yellow, and the quality of products can be improved. According to the heat transfer glue, the scorch retarder is added in the formula, and the pattern transfer printing weather resistance property can be greatly improved. Through the hydroxyethyl heptadecenyl glyoxalidine, the corrosion resistance property can be greatly improved, and the firmness of patterns can be enhanced.