基本信息:
- 专利标题： 高效表达HA蛋白的重组流感病毒及其制备方法和应用
- 专利标题（英）：Recombination influenza virus of high-efficiency-expression HA (hemagglutinin) protein and preparation method and application of recombination influenza virus
- 申请号：CN201210350735.8 申请日：2011-09-08
- 公开(公告)号：CN102851258A 公开(公告)日：2013-01-02
- 发明人： 李泽君 , 滕巧泱
- 申请人： 中国农业科学院上海兽医研究所
- 申请人地址： 上海市闵行区紫月路518号
- 专利权人： 中国农业科学院上海兽医研究所
- 当前专利权人： 中国农业科学院上海兽医研究所
- 当前专利权人地址： 上海市闵行区紫月路518号
- 代理机构： 上海大邦律师事务所
- 代理人： 周东萍
- 分案原申请号： 2011102660820 2011.09.08
- 主分类号： C12N7/01
- IPC分类号： C12N7/01 ; C12N15/63 ; C12N15/44 ; A61K39/145 ; A61P31/16
The invention discloses a PR8 (puerto rico 8) recombination influenza virus. The recombination influenza virus comprises HA (hemagglutinin) and/or NA (neuraminidase) genes of an H4 subtype influenza virus and six internal genes (PB1, PB2, PA, NP, M, NS genes) of the PR8 virus, wherein the NS gene and the NP gene or one of the NS gene and the NP gene comprises the following mutational sites: NS2 protein of NS gene codes comprises E67S site mutation, E74S site mutation or E67S/E74S site mutation, and NP protein of the NP gene codes comprises G132A site mutation. The invention further discloses a preparation method and application of the PR8 recombination influenza virus. The PR8 recombination influenza virus obtained by constructing recombinant plasmids, cell co-transfection and SPF embryonated egg amplification can efficiently express HA protein and/or NA genes and can be used for preparing influenza vaccines on a large scale.