The invention discloses a molecular marker-assisted selection method and primer for Hu-sheep maternal behaviors. The method comprises the following steps of: detecting whether nucleotides at a 284th site and a 339th site of a Hu-sheep PRLR (Prolactin Receptor) gene exon 10 are C or mutant T to determine a gene type of Hu-sheep and selecting a Hu-sheep maternal behavior according to the gene type; if the 284th site and the 339th site are C, determining the gene type of a homozygote to be AA; if the 284th site and the 339th site are T, determining the gene type of the homozygote to be BB; and obtaining the gene type of a heterozygote to be AB, wherein licking and nursing behaviors of the AA gene type are more than those of the AB gene type, and the licking and nursing behaviors of the AB gene type are more than those of the BB gene type; and treading and nursing rejection behaviors of the AA gene type are lower than those of the AB gene type, and the treading and nursing rejection behaviors of the AB gene are lower than those of the BB gene. The method disclosed by the invention utilizes a genetic marker polymorphism to evaluate the Hu-sheep maternal behaviors and can simply and rapidly detect the Hu-sheep maternal behaviors, so that the invention provides a simple and accurate method for evaluating the Hu-sheep maternal behaviors...