基本信息:
- 专利标题： 共转染药物代谢酶和转运体的细胞模型的构建及应用
- 专利标题（英）：Construction and application of cell model for cotransfection of drug metabolic enzyme and transporter
- 申请号：CN201210006590.X 申请日：2012-01-10
- 公开(公告)号：CN102586191A 公开(公告)日：2012-07-18
- 发明人： 曾苏 , 刘瑶 , 胡海红 , 余露山 , 蒋惠娣 , 周慧 , 徐思云 , 王鹭 , 何新
- 申请人： 浙江大学
- 申请人地址： 浙江省杭州市西湖区浙大路38号
- 专利权人： 浙江大学
- 当前专利权人： 浙江大学
- 当前专利权人地址： 浙江省杭州市西湖区浙大路38号
- 代理机构： 杭州求是专利事务所有限公司
- 代理人： 张法高; 赵杭丽
- 生物保藏信息： C2011119 2011.12.15
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C12N15/85 ; C12Q1/02
The invention provides construction of a cell model for cotransfection of CYP3A4 and MDR1. The method comprises the following steps of: cloning P-gp gene to pcDNA3.1(+), transfecting Madin-Darby canine kidney (MDCK) cells, and screening monoclonal cells by using G418, thus obtaining MDCK-MDR1 cell strains; and cloning CYP3A4 to a carrier pcDNA3.1(+)/Hygro which is provided with a hygromycin B selection marker, transfecting MDCK-MDR1 cells, and obtaining the cell model for cotransfection of CYP3A4 and MDR1 through hygromycin B selection. The MDCK-MDR1/CYP3A4 was collected in China Center for Type Culture Collection (CCTCC) on Dec.15, 2011, with the collection number of CCTCC2011119. The model constructed can be used for simulating the absorption and metabolic process of a medicine in the intestinal tract so as to screen the common substrate of P-gp and CYP3A4.