基本信息:
- 专利标题： 拮抗HMGB1Bbox的单克隆抗体及其应用
- 专利标题（英）：Monoclonal antibody having antagonistic action on HMGB1 B box and application thereof
- 申请号：CN201210016550.3 申请日：2012-01-19
- 公开(公告)号：CN102559604A 公开(公告)日：2012-07-11
- 发明人： 苏兆亮 , 周成林 , 孙彩霞 , 孙凡芝 , 刘嫣芳 , 郑东 , 仝佳 , 王胜军 , 许化溪
- 申请人： 江苏大学
- 申请人地址： 江苏省镇江市学府路301号
- 专利权人： 江苏大学
- 当前专利权人： 江苏大学
- 当前专利权人地址： 江苏省镇江市学府路301号
- 代理机构： 南京知识律师事务所
- 代理人： 卢亚丽
- 生物保藏信息： CGMCC No.5534 2011.11.30
- 主分类号： C12N5/20
- IPC分类号： C12N5/20 ; C07K16/18 ; C12R1/91
The invention relates to a hybridoma cell line capable of continuously and stably secreting a monoclonal antibody having antagonistic action on HMGB1 B box, and the secreted antibody. As an important inflammation medium, HMGB1 has proinflammatory and chemotactic functions, and widely takes part in pathogenesis of a plurality of inflammatory diseases, and B box is the biological activity area of HMGB1. The monoclonal antibody prepared with the method has the characteristics as follows: the monoclonal antibody is IgG2a, kappa, and can be specifically combined with vertebrate HMGB1 B box to inhibit the biological activity; furthermore, the monoclonal antibody can be used for western-blot and ELISA tests. In conclusion, the monoclonal antibody can be used for detecting vertebrate HMGB1 B box on one hand, and also can be used for therapy on the other hand.
公开/授权文献:
- CN102559604B 拮抗HMGB1Bbox的单克隆抗体及其应用 公开/授权日：2013-07-17