The invention discloses an automatic test system for a single event effect test of a DC/DC (direct current/direct current) converter. The automatic test system comprises a programmable power supply, a PXI integrated sampling control unit which is connected with an electrical parameter signal control terminal box, an upper PC (personal computer) which is connected with the PXI integrated sampling control unit, and a LabVIEW test software for monitoring an electrical parameter signal and analyzing and processing the electrical parameter signal. The invention also discloses a test method for the automatic test system. By the automatic test system and the test method, the single event effect test data of the DC/DC converter can be tested fully automatically, automatic high-speed data acquisition, automatic product selection, automatic product loading, automatic power setting, test parameter setting, real-time acquired data display and data replay are integrated, the single event effect test efficiency of the DC/DC converter is greatly improved, and the damage to the body of a tester who enters a radiation room repeatedly during the test can be avoided.