The invention relates to a method for detecting a release situation of nicotine in buccal tobacco products, which is characterized by comprising the steps of simulating the release situation of the nicotine in the buccal tobacco products in the human mouth, instantly quantifying the release quantity and the release speed and then detecting the release speed and the release degree of the nicotine in the buccal tobacco products. The release and the collection of the nicotine are realized through a release and collection device in vitro; by means of the device, the release process of the nicotine in the buccal tobacco products in the human mouth can be simulated and nicotine release solutions can be the instantly collected, wherein the release process is a process that the nicotine is continuously extracted from the buccal tobacco products by utilizing artificial saliva at a constant temperature; and the detection of the nicotine content in the release solution is realized through a high performance liquid chromatography. The method has the advantages: the release speed and the release degree of the nicotine in various buccal tobacco products can be rapidly and accurately detected with good repeatability, high sensitivity and short detection period and is suitable for the rapid analysis of mass samples for different industries and different purposes.