
基本信息:
- 专利标题: 一种分离纯化谷氨酰胺转胺酶的方法
- 专利标题(英):Method for separating and purifying transglutaminase
- 申请号:CN201110034437.3 申请日:2011-02-01
- 公开(公告)号:CN102146361A 公开(公告)日:2011-08-10
- 发明人: 王戈莎 , 郭宏明
- 申请人: 泰兴市一鸣生物制品有限公司
- 申请人地址: 江苏省泰州市泰兴市根思乡一鸣路
- 专利权人: 泰兴市一鸣生物制品有限公司
- 当前专利权人: 江苏一鸣生物股份有限公司
- 当前专利权人地址: 江苏省泰州市泰兴市根思乡一鸣路
- 主分类号: C12N9/10
- IPC分类号: C12N9/10
摘要:
本发明提供了一种分离纯化谷氨酰胺转胺酶的方法,它有效地提高了谷氨酰胺转胺酶的比酶活,解决了酶的颜色过深从而影响其应用等问题。本发明仅用一种阳离子交换树脂分离纯化出高纯度的谷氨酰胺转胺酶,方法简单,耗时短,收率高,且可大规模制备,放大到工业生产。
摘要(英):
The invention provides a method for separating and purifying transglutaminase, which effectively increases the specific enzyme activity of the transglutaminase and solves the problems like influence on application due to deep color of the enzyme. In the invention, the transglutaminase with high purity is separated and purified with only one cation exchange resin; and the method has the advantagesof simplicity, low time consumption and high yield, and can be applied to inductiral production for large-scale preparation.
公开/授权文献:
- CN102146361B 一种分离纯化谷氨酰胺转胺酶的方法 公开/授权日:2013-06-19