基本信息:
- 专利标题： 双靶生物传感器细胞试验
- 专利标题（英）：Dual-target biosensor cell assays
- 申请号：CN200980115574.0 申请日：2009-03-04
- 公开(公告)号：CN102037357A 公开(公告)日：2011-04-27
- 发明人： T·A·邦奇 , 方晔 , E·特兰
- 申请人： 康宁股份有限公司
- 申请人地址： 美国纽约州
- 专利权人： 康宁股份有限公司
- 当前专利权人： 康宁股份有限公司
- 当前专利权人地址： 美国纽约州
- 代理机构： 上海专利商标事务所有限公司
- 代理人： 项丹; 崔佳佳
- 优先权： 61/068,266 2008.03.05 US
- 国际申请： PCT/US2009/001369 2009.03.04
- 国际公布： WO2009/111020 EN 2009.09.11
- 进入国家日期： 2010-10-25
- 主分类号： G01N33/50
- IPC分类号： G01N33/50 ; C12Q1/02 ; G01N33/543 ; G01N33/74 ; G01N33/566
Disclosed are multiplexed label-free screening methods involving a biosensor having a live-cell immobilized on its surface, the live-cell having at least two different targets; or involving a biosensor having a mixed population of cells containing two types of cells co-immobilized on a surface of said biosensor; or involving a biosensor having a mixed population of cells containing a first type of cells expressing a first target and a second type of cells expressing a second target, both cell types being co-immobilized on a surface of said biosensor. The target can be a Gi-coupled receptor, a G12/13-coupled receptor, a receptor tyrosine kinase, an ion channel, a sodium-proton exchanger, an integrin receptor, or a transporter. Distinct classes of GPCRs can mediate signaling mostly independently, strongly suggesting that spatial and temporal compartmentalization of signaling cascades is central to GPCR signaling. Most of cellular events downstream of a receptor signaling occur as ''tunneling'' or ''channeling''.