基本信息:
- 专利标题： 在酵母中高效表达N-酰基高丝氨酸内酯酶的DNA及其构建的工程菌
- 专利标题（英）：DNA for high-level expression of N-acyl homoserine lactonase in yeasts and engineering bacteria constructed thereby
- 申请号：CN201010239510.6 申请日：2010-07-27
- 公开(公告)号：CN101914556A 公开(公告)日：2010-12-15
- 发明人： 周志刚 , 张宇婷 , 姚斌 , 曹雅男 , 张美超 , 何夙旭 , 刘玉春 , 孟昆
- 申请人： 中国农业科学院饲料研究所
- 申请人地址： 北京市海淀区中关村南大街12号
- 专利权人： 中国农业科学院饲料研究所
- 当前专利权人： 中国农业科学院饲料研究所
- 当前专利权人地址： 北京市海淀区中关村南大街12号
- 代理机构： 北京纪凯知识产权代理有限公司
- 代理人： 关畅; 任凤华
- 生物保藏信息： CGMCC No.3975 2010.07.02
- 主分类号： C12N15/57
- IPC分类号： C12N15/57 ; C12N9/50 ; C12N15/81 ; C12N15/63 ; C12N1/19 ; C12N5/10 ; C12R1/84
The invention discloses a DNA for high-level expression of N-acyl homoserine lactonase in yeasts and engineering bacteria constructed thereby. The DNA provided by the invention is the DNA expressed by the sequence 2 in a sequence list. On the basis of the traditional homoserine lactonase gene aiiaB546, the DNA sequence thereof is optimized to obtain the optimized gene aiiaB546 M by the method. The expression ability of the optimized gene in Pichia pastoris is obviously higher than that of the gene before optimization. An aiiaB546 M-containing recombinant expression plasmid is constructed; and recombination strains of the high-efficient homoserine lactonase can be obtained by introducing the recombinant expression plasmid into the Pichia pastoris. The expression ability of one of the recombination strains is particularly high. The DNA and the engineering bacteria have great value to the production of the homoserine lactonase.
公开/授权文献:
- CN101914556B 在酵母中高效表达N-酰基高丝氨酸内酯酶的DNA及其构建的工程菌 公开/授权日：2012-06-27