![检测蛋鸡FMO3基因多态性的试剂盒及其方法](/CN/2010/1/51/images/201010255561.jpg)
基本信息:
- 专利标题: 检测蛋鸡FMO3基因多态性的试剂盒及其方法
- 专利标题(英):Kit and method for detecting polymorphism of FMO3 gene of egg-laying chicken
- 申请号:CN201010255561.8 申请日:2010-08-17
- 公开(公告)号:CN101899524B 公开(公告)日:2012-10-03
- 发明人: 潘玉春 , 陈强 , 王起山 , 张向喆 , 杨长锁
- 申请人: 上海交通大学
- 申请人地址: 上海市闵行区东川路800号
- 专利权人: 上海交通大学
- 当前专利权人: 上海交通大学
- 当前专利权人地址: 上海市闵行区东川路800号
- 代理机构: 上海科盛知识产权代理有限公司
- 代理人: 杨元焱
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
The invention relates to a kit and a method for detecting polymorphism of an FMO3 gene of an egg-laying chicken, belonging to the biotechnology field. The kit comprises a forward primer which has a base sequence of 5'-CGGTGTTGAATGATGAC-3', a reverse primer which has a base sequence of 5'-GCATTTGTAGAGGGTAG-3', dNTPs, DNA polymerase and a 10*PCR buffer solution thereof and restriction endonuclease BsrI and a corresponding 10*digestion buffer solution thereof. The invention solves the problem that the allele is difficult to eliminate by using the traditional breeding method, accelerates the breeding process, lowers the breeding cost and has extremely wide application prospects in the selection and breeding work of hybrids of non-fishy smell egg-laying chickens.
公开/授权文献:
- CN101899524A 检测蛋鸡FMO3基因多态性的试剂盒及其方法 公开/授权日:2010-12-01