The invention discloses salmonella enteritidis detection reagent kit and method based on a loop-mediated isothermal amplification technology, wherein two specific inner primers and two specific outer primers are designed by the reagent kit according to the six specific regions of a salmonella enteritidis Sdf I gene conserved region, thereby ensuring the specificity of loop-mediated isothermal amplification and the reliability of a detecting result; in the invention, salmonella enteritidis is detected on the basis of the loop-mediated isothermal amplification technology, a target sequence can be rapidly, efficiently and specifically amplified under an isothermal condition, the operation is simple and convenient, expensive instruments and reagents are not needed, an amplified product is directly developed through fluorescent dye, a result can be judged by naked eyes, and the detection cost is low; and the reagent kit and the method can specifically distinguish serum specific type salmonella enteritidis and other serum type salmonella and are particularly suitable for detection and application in medium and small units and fields.