The invention provides a fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, belonging to the technical field of biology. The method comprises the steps of: 1. adding the ganoderma lucidum spore into water or 20-100% alcohol-containing solution or edible acid solution with the pH value of 3-7 or edible alkali solution with the pH value of 7-10, and evenly stirring to prepare mixture, wherein the weight ratio of the ganoderma lucidum spore to the solution is 1:5-15:2; and 2. processing the mixture under normal temperature though high-pressure pulse electric field to break, wherein the electric-field intensity is 10-110 kilovolt/cm and the number of the pulse is 1-800. By using the high-pressure pulse electric field, the method has less heat energy in the process of breaking, low raised temperature of a breaking target, and short breaking time. The breaking mechanism is not breaking the cell wall in the high-pressure pulse electric field but breaking the cell wall by forming a plurality of through holes on the surface of the cell wall by polar molecules in the solution under the function of the high-pressure pulse electric field, thereby improving the use ratio of bioactive substances.