基本信息:
- 专利标题： HCV基因
- 专利标题（英）：HCV gene
- 申请号：CN200880013943.0 申请日：2008-04-28
- 公开(公告)号：CN101688197A 公开(公告)日：2010-03-31
- 发明人： 森健一 , 槙升 , 深井浩未 , 大植千春
- 申请人： 株式会社先端生命科学研究所
- 申请人地址： 日本埼玉县
- 专利权人： 株式会社先端生命科学研究所
- 当前专利权人： 株式会社先端生命科学研究所
- 当前专利权人地址： 日本埼玉县
- 代理机构： 中国专利代理(香港)有限公司
- 代理人： 吴娟; 敦文洁
- 优先权： 119667/2007 2007.04.27 JP
- 国际申请： PCT/JP2008/058215 2008.04.28
- 国际公布： WO2008/136470 JA 2008.11.13
- 进入国家日期： 2009-10-27
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C07K14/18 ; C12N5/10 ; C12N7/00 ; C12Q1/02 ; C12Q1/68
It is intended to provide a hepatitis C virus gene, a replicon RNA originating in this gene, a replicon-replicating cell having this replicon RNA transferred thereinto and a method of screening a drugby using this replicon-replicating cell. A replicon-replicating cell can be constructed by transferring into a cell a replicon RNA containing: (A) a poLynucleotide which comprises the base sequence represented by SEQ ID NO:5; (B) a poLynucleotide which comprises the base sequence represented by SEQ ID NO:7; (C) a poLynucleotide encoding a polypeptide comprising the amino acid sequence representedby SEQ ID NO:6; (D) a poLynucleotide encoding a polypeptide comprising the amino acid sequence represented by SEQ ID NO:8; or (E) a poLynucleotide comprising a base sequence having a 90% or more homology with the base sequence represented by SEQ ID NO:5 or 7: or a replicon RNA of genotype 1b which contains a nucleotide encoding leucine at the 1804-position and lysine at the 1996-position in the amino acid sequence of hepatitis C virus polyprotein and a poLynucleotide encoding NS4B protein. Use of this replicon-replicating cell makes it possible to screen a drug.