
基本信息:
- 专利标题: 鉴别诊断羊的吕氏泰勒虫和尤氏泰勒虫的试剂盒及方法
- 专利标题(英):Kit and method for differential diagnosis of Theileria luwenshuni and T.uilenbergi in goats
- 申请号:CN200910147073.2 申请日:2009-06-01
- 公开(公告)号:CN101565754A 公开(公告)日:2009-10-28
- 发明人: 殷宏 , 罗建勋 , 关贵全 , 马米玲 , 刘爱红 , 刘志杰 , 高金亮 , 党志胜 , 李有全 , 任巧云 , 刘军龙 , 牛庆丽
- 申请人: 中国农业科学院兰州兽医研究所
- 申请人地址: 甘肃省兰州市城关区徐家坪1号
- 专利权人: 中国农业科学院兰州兽医研究所
- 当前专利权人: 中国农业科学院兰州兽医研究所
- 当前专利权人地址: 甘肃省兰州市城关区徐家坪1号
- 代理机构: 兰州振华专利代理有限责任公司
- 代理人: 张晋
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12R1/90
The invention relates to a kit for detecting whether the goat infects the Theileria luwenshuni or T.uilenbergi, a preparing method and applying method for the kits. The kit comprises at least two pairs of specific primers for the Theileria luwenshuni and the T.uilenbergi. The kit application method comprises: extracting DNA from the goat blood sample to be detected, mixing with the polyase, buffer, specific primers for the Theileria luwenshuni or the T.uilenbergi, amplifying, taking the amplification product and TAE buffer, performing electrophoresis detection in the agarose gel, finding out whether the detected goat suffers from the Theileria luwenshuni diease or T.uilenbergi disease based on the specific electrophoresis band form in the specific electrophoresis channel.