The invention discloses an electro-transformation method for inducing a constructed plasmid containing exogenous genes to acidithiobacillus caldus. The method comprises the following steps: the constructed plasmid containing the exogenous genes is extracted and the concentration of the plasmid is corrected; the competent cells of the acidithiobacillus caldus are cultured and collected; the electro-transformation and plate screening are carried out, and positive transformants are identified and detected; wherein, the electro-transformation parameters are as follows: the voltage is 10,500V/cm, the resistance is 400 omega and the capacitance is 25muF. By adopting the method, the electro-transformation of the acidithiobacillus caldus is realized for the first time, and a recovery culture scheme and a screening method for the cidithiobacillus caldus are established, which provides a convenient, quick and high-efficiency gene delivery method for the studies of genetic engineering breeding, genetics and molecular biology of the acidithiobacillus caldus.