An isolating manifold fluidly connected between a hydraulic valve and a hydraulic manifold operates to automatically isolate the valve from the hydraulic manifold as the valve is removed from the manifold, without requiring any particular blocking and bleeding procedures to be implemented on the valve or the manifold prior to the removal process The isolating manifold includes an adaptor removably mounted onto a base with various pressure, control and tank fluid channels disposed in alignment through the adaptor and base portions to allow fluid to flow through each of the channels between the hydraulic manifold and the valve. During operation, the valve is mounted onto the adaptor while the base is mounted onto the hydraulic manifold. The valve may be removed from the hydraulic manifold by removing the adaptor from the base, during which process, the isolating manifold operates automatically to block the pressure and control ports of the hydraulic manifold and to bleed pressurized fluid from the pressure and control channels of the valve to a tank channel so as to automatically isolate the valve from the hydraulic manifold as the valve is removed from the hydraulic manifold.