基本信息:
- 专利标题： 用于增加细胞培养物的寿命和蛋白收率的改良方法和组合物
- 专利标题（英）：Improved methods and compositions for increasing longevity and protein yield from a cell culture
- 申请号：CN200680035226.9 申请日：2006-07-14
- 公开(公告)号：CN101273121A 公开(公告)日：2008-09-24
- 发明人： D·M·戈登伯格 , Z·屈 , C·H·张 , E·A·罗西 , J·-D·杨 , 黛安·诺德斯特龙
- 申请人： 免疫医疗公司
- 申请人地址： 美国新泽西州
- 专利权人： 免疫医疗公司
- 当前专利权人： 免疫医疗公司
- 当前专利权人地址： 美国新泽西州
- 代理机构： 中国专利代理(香港)有限公司
- 代理人： 刘冬; 梁谋
- 优先权： 11/187,863 2005.07.25 US
- 国际申请： PCT/US2006/027820 2006.07.14
- 国际公布： WO2007/015848 EN 2007.02.08
- 进入国家日期： 2008-03-24
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C12N5/06 ; C12N15/00
Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells.
公开/授权文献:
- CN101273121B 用于增加细胞培养物的寿命和蛋白收率的改良方法和组合物 公开/授权日：2012-07-04