The invention relates to a pyrryl modified bi-functional polymeric adsorbent with the structural unit shown in the drawing, wherein R is shown in the drawing. The specific surface area of the bi-functional polymeric adsorbent is 50 to 1600 m<2>/g, the pore volume is 0.01-1.0 cm<3>/g, and the exchange capacity is 0.1-5.0 mmol/g. The preparation method for the resin is that chloromethylated beads are swelled in solvents such as nitrobenzene, etc. to add catalyst to obtain polystyrene-divinyl benzene adsorbent; reaction mother solution is exhausted and residual solvent and catalyst are washed away; swelling is carried out by adding in the functionalized pyrryl agent, so as to further react with the residual chloromethyl on the internal and external surfaces of the resin to obtain the pyrryl modified bi-functional polymeric adsorbent. The resin has dual functions of absorbing and ion exchanging (coordination). The resin also has hydrophilic property, alkali pyrryl functional groups and hydrophobic styrene frame and has excellent affinity for amphoteric bioreagents such as protein; and the acid-base interaction between the pyrryl functional groups and acid organic materials is particularly in favor of the separation of acid organic materials.