基本信息:
- 专利标题： 一种重组葡激酶及其高效分泌表达的方法
- 专利标题（英）：Recombination staphylokinase and highly effective secretion expression method thereof
- 申请号：CN200710053340.0 申请日：2007-09-20
- 公开(公告)号：CN101240284A 公开(公告)日：2008-08-13
- 发明人： 乐超银 , 刘敏 , 郭政红 , 谢伟 , 梁薇 , 姚伟 , 梁宏伟 , 陈发菊 , 陈凡 , 戴建武
- 申请人： 三峡大学
- 申请人地址： 湖北省宜昌市大学路8号
- 专利权人： 三峡大学
- 当前专利权人： 三峡大学
- 当前专利权人地址： 湖北省宜昌市大学路8号
- 代理机构： 宜昌市三峡专利事务所
- 代理人： 成钢
- 主分类号： C12N15/57
- IPC分类号： C12N15/57 ; C12N9/48 ; C12N1/21 ; C12R1/19
A staphylococci kinase and a method for high efficiency secreting and expressing recombination staphylococci kinase in biotechnology are provided. It obtains glucose kinase (Sak) containing signal peptide by using total DNA of staphylococcus aureus as form through PCR amplification. The 33th, 34th and 36th bite amino acid of the gene order are different from the reported Sak gene. Connecting the gene to carrier Pgex-4T-1, then transforming host bacterium to obtain recombination engineering bacterium. The recombination engineering bacterium can secreted express recombination glucose kinase having the function of fiber dissolvedzymogen activation by IPTG induction. The recombination glucose kinase expressed in the method of the invention has high gene expression and expressed outcome mainly is secretion type albumen which exists in thalli outer cytoplasm or outer cell. Thus, purifying process is simple, yield is high and production cost is low.
公开/授权文献:
- CN101240284B 一种重组葡激酶及其高效分泌表达的方法 公开/授权日：2012-05-30