基本信息:
- 专利标题： NUCLEIC ACID HYBRIDIZATION METHODS
- 申请号：CA3136747 申请日：2020-05-01
- 公开(公告)号：CA3136747A1 公开(公告)日：2020-11-05
- 发明人： ARSLAN SINAN , HE MOLLY , PREVITE MICHAEL
- 申请人： ELEMENT BIOSCIENCES INC
- 专利权人： ELEMENT BIOSCIENCES INC
- 当前专利权人： ELEMENT BIOSCIENCES INC
- 优先权： US201962841541 2019-05-01
- 主分类号： C12Q1/6813
- IPC分类号： C12Q1/6813 ; C12Q1/68 ; C12Q1/6806 ; C12Q1/6876 ; G01N33/53
摘要：
Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one polar, aprotic, organic solvent, and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.