基本信息:
- 专利标题： Cation mediated triplex hybridization assay
- 申请号：AU2001280007 申请日：2001-07-09
- 公开(公告)号：AU2001280007B2 公开(公告)日：2005-03-24
- 发明人： ERIKSON GLEN H , PICARD PIERRE , DAKSIS JASMINE I
- 申请人： INGENEUS CORP
- 专利权人： INGENEUS CORP
- 当前专利权人： INGENEUS CORP
- 优先权： US61326300 2000-07-10
- 主分类号： G01N21/64
- IPC分类号： G01N21/64 ; C12N15/09 ; C12Q1/68 ; G01N21/78 ; G01N33/483 ; G01N33/53 ; G01N33/542 ; G01N33/566 ; G01N33/58 ; G01N37/00 ; C12Q001/68 ; C07H021/04 ; G01N015/06 ; C12M001/36
摘要：
Triplex complexes contain a single-stranded probe bound to a double-stranded nucleic acid target, in which the probe includes a heteropolymeric nucleic acid or a heteropolymeric nucleic acid analog. All base triplets of the complex are members selected from the group consisting of A-T-A, T-A-T, U-A-T, T-A-U, A-U-A, U-A-U, G-C-G and C-G-C. A cation-facilitated assay includes detecting the presence of such triplex complexes to determine the degree of complementarity between the probe and target sequence. The assay preferably detects a change in fluorescent intensity of a label as a function of binding affinity between the probe and target. The label can be covalently tethered to the probe or to the target, or can be an intercalating fluorophore in the reaction medium.
信息查询:
EspacenetIPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N21/00 | 利用光学手段，即利用红外光、可见光或紫外光来测试或分析材料 |
| --------G01N21/01 | .便于进行光学测试的装置或仪器 |
| ----------G01N21/63 | ..光学激发的 |
| ------------G01N21/64 | ...荧光；磷光 |