专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

IL290678A Novel crispr enzymes and systems 未知
公开(公告)号：IL290678A
公开(公告)日：2022-04-01
申请号：IL29067822
申请日：2022-02-16
申请人： HARVARD COLLEGE , MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , FENG ZHANG , BERND ZETSCHE , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
发明人： FENG ZHANG , BERND ZETSCHE , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.

2. 发明专利

IL281159D0 Novel crispr enzymes and systems 未知
公开(公告)号：IL281159D0
公开(公告)日：2021-04-29
申请号：IL28115921
申请日：2021-02-28
申请人： BROAD INST INC , MASSACHUSETTS INST TECHNOLOGY , HARVARD COLLEGE , FENG ZHANG , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH , SOUMYA KANNAN
发明人： FENG ZHANG , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH , SOUMYA KANNAN
IPC分类号： C12N9/22 , C12N9/78
摘要： The present disclosure provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides mutated Cas13 proteins and their use in modifying target sequences as well as mutated Cas13 nucleic acid sequences and vectors encoding mutated Cas13 proteins and vector systems or CRISPR-Cas13 systems.

3. 发明专利

IL276349D0 Crispr effector system based diagnostics 未知
公开(公告)号：IL276349D0
公开(公告)日：2020-09-30
申请号：IL27634920
申请日：2020-07-28
申请人： MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , HARVARD COLLEGE , FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
发明人： FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
IPC分类号： C12N9/08 , C12N9/22 , C12Q1/6827
摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences, and includes detection by colorimetric and/or fluorescence shifts. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

4. 发明专利

IL275597D0 Crispr effector system based multiplex diagnostics 未知
公开(公告)号：IL275597D0
公开(公告)日：2020-08-31
申请号：IL27559720
申请日：2020-06-22
申请人： MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , HARVARD COLLEGE , FENG ZHANG , BERND ZETSCHE , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
发明人： FENG ZHANG , BERND ZETSCHE , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
IPC分类号： C12N9/22 , C12Q1/6804 , C12Q1/6823
摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

5. 发明专利

IL256369D0 Novel crispr enzymes and systems 未知
公开(公告)号：IL256369D0
公开(公告)日：2018-02-28
申请号：IL25636917
申请日：2017-12-17
申请人： HARVARD COLLEGE , MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , FENG ZHANG , BERND ZETSCHE , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
发明人： FENG ZHANG , BERND ZETSCHE , IAN SLAYMAKER , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
IPC分类号： C12N20060101
摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.

6. 发明专利

IL273360D0 Crispr effector system based diagnostics 未知
公开(公告)号：IL273360D0
公开(公告)日：2020-05-31
申请号：IL27336020
申请日：2020-03-17
申请人： HARVARD COLLEGE , MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
发明人： FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH
IPC分类号： B01L3/00 , C12Q1/6816
摘要： Provided herein is a lateral flow diagnostic device and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The first end may further comprise a first region loaded with a detectable ligand, a CRISPR effector system, a detection construct, a first test band comprising a biotin ligand, and a second test band comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate towards the first and second capture regions, thereby generating a detectable signal, which may be indicative of a disease state.

7. 发明专利

ZA201504446B 81-FACET DIAMOND WITH 10-HEART-AND-10-ARROW STRUCTURE INSIDE 未知
公开(公告)号：ZA201504446B
公开(公告)日：2016-11-30
申请号：ZA201504446
申请日：2015-06-19
申请人： FENG ZHANG A CHINESE CITIZEN , KUNZHI ZHANG A CHINESE CITIZEN
发明人： FENG ZHANG , KUNZHI ZHANG
摘要： The present invention relates to an 81-facet diamond with a 10-heart-and-10-arrow structure inside. The diamond comprises 10 main crown facets and 10 main pavilion facets.The diamond further comprises one table facet. Crown star facets are disposed at places where the main crown facets are connected to the table facet, and the number of the crown star facets is 10. Crown small facets are disposed at places wherein main crown facet edges are connected to the crown star facets, and the number of the crown small facets is 10. Small sector facets are disposed at places where the main crown facets are connected to the crown small facets, and the number of the small sector facets is 20. Auxiliary facets of the main pavilion facets are disposed at main pavilion facet connection places, and the number of the auxiliary facets of the main pavilion facets is 20. The present invention has the following beneficial effects: the diamond is cut into 81 facets, and a 10-heart-and-10-arrow structure is provided inside, higher quality and value are provided for the diamond by using a high-end cutting process, and the brightness of the diamond under sunshine is increased by 20 to 30%. The diamond according to the present invention has a higher brilliant degree.

8. 发明专利

IL284808B Crispr enzyme mutations reducing off-target effects 未知
公开(公告)号：IL284808B
公开(公告)日：2022-07-01
申请号：IL28480821
申请日：2021-07-12
申请人： BROAD INST INC , MASSACHUSETTS INST TECHNOLOGY , FENG ZHANG , LINYI GAO , BERND ZETSCHE , IAN SLAYMAKER
发明人： FENG ZHANG , LINYI GAO , BERND ZETSCHE , IAN SLAYMAKER
IPC分类号： A61P11/00 , A61P17/00 , A61P17/06 , A61P21/00 , A61P25/16 , A61P25/18 , A61P25/24 , A61P25/28 , A61P27/02 , A61P29/00 , A61P31/00 , A61P31/12 , A61P31/18 , A61P31/20 , A61P35/00 , A61P37/06 , C12N15/10 , C12N15/11 , C12N15/90 , G09B23/28
摘要： Disclosed and claimed are mutation(s) or modification(s) of the CRISPR enzyme, for example a Cas enzyme such as a Cas9, which obtain an improvement, for instance a reduction, as to off-target effects of a CRISPR-Cas or CRISPR-enzyme or CRISPR-Cas9 system or complex containing or including such a mutated or modified Cas or CRISPR enzyme or Cas9. Methods for making and using and uses of such mutated or modified Cas or CRISPR enzyme or Cas9 and systems or complexes containing the same and products from such methods and uses are also disclosed and claimed.

9. 发明专利

IL256368A Crispr enzyme mutations reducing off-target effects 未知
公开(公告)号：IL256368A
公开(公告)日：2021-07-29
申请号：IL25636817
申请日：2017-12-17
申请人： BROAD INST INC , MASSACHUSETTS INST TECHNOLOGY , FENG ZHANG , LINYI GAO , BERND ZETSCHE , IAN SLAYMAKER
发明人： FENG ZHANG , LINYI GAO , BERND ZETSCHE , IAN SLAYMAKER
IPC分类号： C12N9/22 , C12N15/11 , C12N15/90
摘要： Disclosed and claimed are mutation(s) or modification(s) of the CRISPR enzyme, for example a Cas enzyme such as a Cas9, which obtain an improvement, for instance a reduction, as to off-target effects of a CRISPR-Cas or CRISPR-enzyme or CRISPR-Cas9 system or complex containing or including such a mutated or modified Cas or CRISPR enzyme or Cas9. Methods for making and using and uses of such mutated or modified Cas or CRISPR enzyme or Cas9 and systems or complexes containing the same and products from such methods and uses are also disclosed and claimed.

10. 发明专利

IL279065D0 Crispr effector system based amplification methods, systems, and diagnostics 未知
公开(公告)号：IL279065D0
公开(公告)日：2021-01-31
申请号：IL27906520
申请日：2020-11-29
申请人： HARVARD COLLEGE , MASSACHUSETTS INST TECHNOLOGY , BROAD INST INC , FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH , MAX KELLNER
发明人： FENG ZHANG , JONATHAN S GOOTENBERG , OMAR O ABUDAYYEH , MAX KELLNER
IPC分类号： C12Q1/6844
摘要： Provided herein are methods and systems for amplifying and/or detecting target double-stranded or single-stranded nucleic acids. The methods comprise combining a sample comprising the target nucleic acid with an amplification reaction mixture, amplifying the target nucleic acid, and further amplifying the target nucleic acid by repeated opening, unwinding, annealing and extension under isothermal conditions. The amplification reaction mixture may include an amplification CRISPR system, a helicase, a primer pair, and a polymerase. The amplification CRISPR system may comprise a first and second CRISPR/Cas complex, the first and second CRISPR/Cas complex may comprise a first CRISPR/Cas enzyme and a first guide molecule that guides the first CRISPR/Cas complex to a first strand of the target nucleic acid; the second CRISPR/Cas complex may comprise a second CRISPR/Cas enzyme and a second guide molecule that guides the second CRISPR/Cas complex to a second strand of the target nucleic acid.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式